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KMID : 0363420070210020097
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Journal of Korean Oriental Pediatrics
2007 Volume.21 No. 2 p.97 ~ p.107
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Inhibitory Effects of Jojoongikgi-tang on the Secretion of PGE2 and NO and Cytokines in LPS-stimulated BV2 Microglial Cells
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Lee Jun-Moon
Lyu Sun-Ae
Lee Seung-Yeon
Kim Bo-Kyung
Ko Woo-Shin
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Abstract
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Objectives:This experiment is about Jojoongikgi-tang(JIT) exerts anti-inflammatory effects in BV2 microglial cells, and the effect of JIT on Nitric oxide(NO) production in lipopolysaccharide(LPS)-stimulated BV2 microglial cells were also demonstrated.
Methods: To investigate the anti-inflammatory effects of JIT, NO production, expression level of iNOS mRNA, PGE2 synthesis, expression COX-2 mRNA, cell viability, $TNF-{\alpha}$ mRNA expression were examined.
Results: The expression level of inducible nitric oxide synthase(iNOS) was decreased by JIT, and the production of Prostaglandin E2(PGE2) and the expression of Cox-2 mRNA also were inhibited by JIT. Proinflammatory mediators, such as $TNF-{\alpha}$, $IL-1{\beta}$, IL-12, were inhibited by JIT in a dose-dependent manner.
Conclusion:JIT have anti-inflammatory effects in BV2 microglial cells and could be used in inflammatory disease.
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KEYWORD
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Jojoongikgi-tang(JIT), NO, COX-2, cytokines
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